Morphology (Haematological Cytology)

Screening process

We perform a comprehensive screening assessment on almost every sample received:

1. Initial morphological screening

• Uses modified Wright's stain

• Performed by the HODS duty scientist

• May provide a diagnosis or guide further investigations (e.g. choosing relevant flow cytometry and/or FISH panels or molecular tests)

• Assesses sample quality, cellularity, and integrity

2. Bone marrow aspirate analysis

• Reported by a consultant haematologist

• Double-reported by another consultant when appropriate

• Includes differential count on peripheral blood and/or bone marrow aspirate slides when needed

• Assesses cellularity and presence of myelodysplasia or other abnormal cells

3. Additional testing

• Iron stain (Perls' Prussian blue) performed if indicated

This screening process helps us determine the most appropriate diagnostic pathway for each sample.

Back To Top

Appropriate specimens

We accept the following specimens for haematopathology diagnosis:

• Blood smear (unstained)

• Bone marrow smears (3 slides) and/or squash preparations (unstained)

• Bone marrow aspirate (1-2 mL in EDTA)

• Dab preparations from trephine biopsy or solid tissue (e.g. lymph node)

• Body fluids in conservation fluid (e.g. cerebrospinal fluid (CSF), pleural fluid)

Sample preparation and storage

Prepare and store samples as follows:

• Air-dry all slides thoroughly

• Store at room temperature

Important

Inadequately dried samples often stain abnormally and may not be suitable for full morphological assessment.

Bone marrow aspirate reporting

Our comprehensive bone marrow aspirate reports include:

• Description and quantification of cell populations

• Detailed description of abnormal cell types

• Quantification of myelodysplasia per cell line, when indicated

• Results of iron stain (Perls' Prussian Blue), if performed

• Interpretation of morphological results

• Reference to most relevant pending investigations

Important

Always include blood films with bone marrow aspirate samples.

Back To Top